earle’s balanced salt solution (ebss (Thermo Fisher)
Structured Review

Earle’s Balanced Salt Solution (Ebss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/earle's+balanced+salt+solution+(ebss/pmc11801289-59-6-11
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo"
Article Title: Enhanced autophagic clearance of amyloid-β via histone deacetylase 6-mediated V-ATPase assembly and lysosomal acidification protects against Alzheimer’s disease in vitro and in vivo
Journal: Neural Regeneration Research
doi: 10.4103/NRR.NRR-D-23-01633
Figure Legend Snippet: VPA activates autophagy and promotes lysosomal function in an in vitro model of Alzheimer’s disease. (A) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with EBSS, and mRFP-GFP-LC3 fluorescent signals were detected by confocal microscopy. Scale bar: 5 μm. (B, C) mRFP-positive and GFP-positive puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). (D) Detection of mRFP-GFP-LC3 delivery to lysosomes. SH-SY5Y cells (WT), VEH-treated APP swe cells, and VPA-treated APP swe cells were treated with Baf A1. Scale bar: 10 μm. (E, F) mRFP and GFP puncta were quantified to determine the number of autophagosomes and autolysosomes per cell ( n = 6 randomly selected fields). * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey’s post hoc test). Baf A1: Bafilomycin A1; EBSS: Earle’s Balanced Salt Solution; mRFP-GFP-LC3: monomeric red fluorescence protein–enhanced green fluorescence protein-microtubule-associated protein 1 light chain3; VEH: vehicle; VPA: valproic acid.
Techniques Used: In Vitro, Confocal Microscopy, Fluorescence
Related Articles
Microscopy:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using Western Blot:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using Inhibition:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using Control:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using In Vitro:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using Cell Culture:Article Title: Protein Arginine Methyltransferases 5 (PRMT5) affect Multiple Stages of Autophagy and Modulate Autophagy-related Genes in Controlling Breast Cancer Tumorigenesis. Article Snippet: Background: Autophagy disorders are linked to human cancer, and the details of their mechanisms remain unclear.. Objective: To investigate the regulatory role of PRMT5 in the autophagy of breast cancer cells.. Methods: Human breast adenocarcinoma cell lines (MDA-MB-231, MCF7) were cultured. Article Title: CDKL5 regulates p62-mediated selective autophagy and confers protection against neurotropic viruses Article Snippet: For amino-acid starvation, cells were treated for either 1h (GFP-LC3 microscopy assays) or 3 h (LC3 I/II western blots) with Article Title: Reduced Palmitoylation of <scp>SQSTM1</scp>/p62 in Huntington Disease Is Associated With Impaired Autophagy Article Snippet: Where indicated, induction of autophagy in HeLa cells was conducted by the application of Article Title: Binding epitope for recognition of human TRPM4 channel by monoclonal antibody M4M Article Snippet: In brief, fetal brains from embryonic day 18 (E18) pregnant transgenic rats were obtained and digested for 40 min in Article Title: Long‑chain non‑coding RNA GAS5 promotes cell autophagy by modulating the miR‑181c‑5p/ ATG5 and miR‑1192/ ATG12 axes. Article Snippet: For the determination of macrophage autophagy in vitro, RAW264.7 cells were cultured in Article Title: GCN2 and perk kinase inhibitors and methods of use thereof Article Snippet: The next day each well was replaced with 1 mL Earle's Balanced Salt Solution (EBSS, Invitrogen, Carlsbad, CA) supplemented with 10% Dialyzed Fetal Bovine Serum, 5.5 mM Glucose, 1% Penicillin/Streptomycin and 1% Article Title: Modeling bile duct ischemia and reoxygenation injury in human cholangiocyte organoids for screening of novel cholangio-protective agents Article Snippet: In brief, donor liver tissue was minced, rinsed twice with Earle's balanced salt solution (EBSS, Thermo-Fisher, Waltham, USA), and digested in collagenase solution (2.5 mg/mL collagenase Type A, Sigma–Aldrich, St. Louis, USA) in Article Title: Zwitterionic poly(carboxybetaine methacrylate) (polyCBMA) decreases desiccating damage to corneal epithelial cells Article Snippet: For cell experiments employing the desiccative culture conditions, polyCBMA was dissolved and diluted using |